New Graduate Lab Survival Guide! Extraction of pro, Brezina

7. After the ultrasound The cells were centrifuged at 12000 rpm for 25 min in a 4 degree centrifuge. 8. The centrifuged supernatant was sub-packaged and transferred to 1.5ml centrifuge tubes were stored at -80 ℃ for later use. 2 Extraction of total protein from tissue: 1. Place a small amount of tissue mass in Add 500ul of strong RIPA lysate containing PMSF into a 1.5ml centrifuge tube, first use clean scissors to cut the tissue block as much as possible, when the tissue is soft (such as brain tissue), then use a 1ml gun head to continuously blow and suck, then use a homogenizer to further break the tissue, and finally use an ultrasonic instrument to carry out ultrasonic lysis; When the tissue is tough (such as muscle tissue), the homogenizer can be directly used to break the tissue, and finally the ultrasonic instrument can be used to carry out ultrasonic lysis. (Dissecting tissue is to make tissue pieces smaller and smaller by different methods.). 2. Same method as above ? Put the centrifuge tube into a 4 degree centrifuge, centrifuge at 12000 rpm for 25 min,wiped film distillation, and take the supernatant into a 1.5ml centrifuge tube and store it at -80 ℃. Ok, that's all for today. To be continued.. "Medical School" has always been committed to serving "medical scholars", pushing the most cutting-edge and valuable original articles on clinical and scientific research to clinicians and researchers. Return to Sohu to see more. Responsible Editor:. toptiontech.com